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Typer Viewer - Now Available
Sequenom is pleased to announce the launch of the new MassARRAY® Typer Viewer software, which supports a subset of the Typer Analyzer functions. The MassARRAY Typer Analyzer software package is a powerful suite of applications for SNP genotyping using hME and iPLEX® chemistries. It provides the setup and analysis modules necessary to perform genotyping assays on the MassARRAY system and allows users to visualize and analyze their genotyping data generated by service providers on the MassARRAY system - even without a connection to the MassARRAY database.
For additional product information, please contact your local Sequenom office.
Articles of Interest:
Flatscher-Bader, T. et al. 2011. Increased de novo copy number variants in the offspring of older males. Transl. Psychiatry e34.
A mouse model was used to explore the hypothesis that the offspring of older males have an increased risk of de novo CNVs. The offspring were assessed on selected behavioral tests and structural MRI to determine the correlation between the identified CNVs and phenotypic behaviors. Below are the findings:
- 7 distinct CNVs were identified by CGH microarrays in a set of 12 offspring and their parents.
- Competitive quantitative CNV assays on the MassARRAY system confirmed all of those CNVs in the original set. The CNVs were also confirmed in an independent set of 77 offspring and their parents. The quantitative nature of the Sequenom CNV assays made it possible to establish the associated frequencies of those CNVs.
Two of those CNVs were associated with Auts2 (autism susceptibility candidate 2) and genes linked to schizophrenia, autism, and brain developments, indicating that the offspring of older fathers have an increased risk of neurodevelopmental disorders such as schizophrenia and autism.
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Guo et al., Epigenetic mechanisms of amyloid-β production in anisomycin-treated SH-SY5Y cells
Recent studies have indicated that epigenetic mechanisms may play a pivotal role in Alzheimer disease (AD) pathogenesis and development. This study investigates epigenetic mechanisms such as DNA methylation and histone acetylation involved in the activation of stress-related signaling pathways for amyloid-β (Aβ) production, a pathological hallmark of AD:
- SH-SY5Y cells were treated with anosimycin, an activator of stress-related mitogen-activated protein kinase (MAPK), leading to a significant increase of cellular amyloid-β levels.
- qRT-PCR and Western blot analysis showed that the genes APP, BACE1 and PS1 were up-regulated.
- EpiTYPER analysis showed that this was due to demethylation in the gene promoters.
- It is concluded that stress-induced amyloid-β production could be due to DNMT-dependent hypomethylation and increased histone H3 acetylation leading to increased transcription of APP, BACE1 and PS1.
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The MassARRAY system, EpiTYPER assay, MelaCarta Panel and IPLEX Gold assay are for Research Use Only. Not for use in diagnostic procedures. |
Upcoming Events:
Name: International Congress of Human Genetics (ICHG)
Location: Montreal Convention Center (Le Palais des Congres)
Montreal, Quebec, Canada
Dates: October 11th - 15th
Details: Come visit us at Booth # 309
Website: http://www.ichg2011.org/
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